Biochem/physiol Actions
Reversible: no
Product does not compete with ATP.
Cell permeable: yes
Target IC50: 20 nM as antagonist of the Shh (Sonic Hedgehog) signaling pathway in the Shh-LIGHT2 assay and in Ptch1-l- cells
Primary TargetShh (Sonic Hedgehog) signaling pathway
General description
A potent, cell-permeable antagonist of the Shh (Sonic Hedgehog) signaling pathway (IC50 = 20 nM in the Shh-LIGHT2 assay and in Ptch1-l- cells) by binding directly to Smoothened (Smo; Kd = 1.2 nM), a distant relative of G protein-coupled receptors. Unlike cyclopamine (Cat. No. 239803), SANT-1 equipotently inhibits the activities of both wild-type and oncogenic Smo (IC50 = 30 nM in SmoA1-LIGHT2 assay).
A potent antagonist of the Sonic Hedgehog (Shh) signaling pathway (IC50 = 20 nM in Shh-LIGHT2 assay and in Ptch1-l-< cells) that acts by binding to Smoothened (Smo; KD = 1.2 nM), a distant relative of G protein-coupled receptors. In contrast to cyclopamine, SANT-1 inhibits the activities of both wild type and oncogenic Smo with equal potency (IC50 = 30 nM in SmoA1-LIGHT2 assay).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 14071.Frank-Kamenetsky, M., et al. 2002. J. Biol.1, 10.
Packaging
Packaged under inert gas
5 mg in Plastic ampoule
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.r>r>r>r>From Catalog:r>Desc. Field- added "cell-permeable"
Warning
Toxicity: Standard Handling (A)
This product has met the following criteria to qualify for the following awards: